为了高分辨率分离游离氨基酸,请选择Thermo Scientific™Dionex™AminoPac™PA10氨基酸色谱柱。Dionex AminoPac PA10色谱柱填充有疏水,聚合,薄膜,阴离子交换树脂,该树脂在pH 0-14的范围内稳定。独特的pH稳定性允许使用有助于金电极上氨基酸阳极氧化的洗脱液。建议将该色谱柱与Thermo Scientific™Dionex™AAA-Direct™氨基酸分析仪系统一起使用,该系统可通过集成的脉冲安培检测(IPAD)直接检测一级和二级氨基酸,而无需柱前或柱后衍生化。
Optimized for Separation of Free Amino Acids by the Dionex AAA-Direct System
The Dionex AAA-Direct system eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.
Engineered for Superior Chromatographic Performance
Carbohydrates and amino acids separated and detected simultaneously
Mid-femtomole to low-picomole detection limits
Compatible with all commonly used hydrolysis procedures
Can be configured for on-line analysis
About Dionex AminoPac Amino Acid Columns
Thermo Scientific offers two approaches to amino acid analysis: anion-exchange separation by the Dionex AAA-Direct system, in which amino acids are detected directly without the need for derivatization; and anion-exchange separation with postcolumn derivatization.
Dionex AminoPac PA10 columns separate free amino acids without the need for derivatization.
Dionex AminoPac PA10 columns separate free amino acids with derivatization.
Both columns are compatible with solvent, high pH, and high temperatures.
Both columns facilitate easy scale-up.
Dionex AminoPac columns are used for high-resolution separations of free amino acids as well as for separation and detection of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids.
粒径 | 8.5 µm |
固定相 | AminoPac PA10 |
类型 | Microbore Separator |
色谱柱容量 | 60 µeq |
流速 | 0.25 mL/min. |
最大压力 | 4000 psi (275 bar) |
pH | 0 to 14 |
长度(公制) | 250 mm |
直径(公制) | 2 mm |
温度(公制) | 30°C |
孔径 | <10 Å |
最大温度(公制) | 40°C |
乳胶交联 | 30 to 40% |
乳胶直径(公制) | 180 nm |
交联 | 55% DVB |
柱结构 | PEEK with 10-32 Threaded Ferrule-Style End Fittings. All Components Are Nonmetallic. |
流动相兼容性 | pH 0 to 14, 100% Compatible with Common Organic Solvents, Sodium Acetate Ionic Form Eluents Only |
阴离子交换容量 | 60 µeq/Column |
溶剂 | 100% Compatible with Common Organic Solvents |
树脂组成 | 8.5 µm Diameter Microporous (<10 Angst;) Bead (55% Cross-Linked) Agglomerated with an 80 nm Microbead Alkyl Ammonium Functionalized Latex (1% Cross-Linked). |
适用于(设备) | AAA-Direct System |
Unit Size | Each 1 |
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